Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific HEPES Buffered Saline Solution 2X, 500 Milliliters
HEPES Buffered Saline Solution 2X is a sterile, concentrated buffer solution used in biological and biochemical applications where stable pH control is required. The 2X concentration provides a convenient way to dilute the solution to the desired pH range for specific experiments, such as protein analysis, enzyme assays, and cell culture maintenance. HEPES is a widely used buffer in cell and molecular biology due to its minimal interference with biological processes and its ability to maintain a stable pH in a variety of biological environments. The 500mL bottle size is ideal for routine laboratory use in large-scale research and clinical applications.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005313 TRIS HYDROCHLORIDE 100G
Dojindo Molecular Technologies Inc EPPS ( 25G), Good's buffer, CAS: 16052-06-5, pka=8.00, Active pH 7.5-8.5, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Biotang Inc RNA Sample Loading Buffer (No Eth Bromide), 1 ml
RNA Sample Loading Buffer (No Eth Bromide), 1 ml.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 10 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660679.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 10 L
TBE 10X Solution (TRIS Borate EDTA) is a powerful buffering solution designed for use in electrophoresis, particularly for separating nucleic acids such as DNA and RNA. This 10-liter bottle provides a concentrated formula of Tris, borate, and EDTA, which maintain pH and ionic strength during electrophoretic separation. The TBE buffer ensures consistent and effective separation of nucleic acid samples by maintaining a stable environment for the molecules to migrate through the gel matrix. This large 10-liter container is ideal for laboratories requiring bulk quantities for high-throughput electrophoresis or other molecular biology techniques.
Biotang Inc TRIS- Tricine -SDS Running Buffer (10X)(Anode Buff), 500 ml
TRIS- Tricine -SDS Running Buffer (10X)(Anode Buff), 500 ml.
eMolecules 502650-56-8 | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | 502650-56-8 | 172.224 | C9H16O3
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Gold Biotechnology Inc Tris HCl 500 g
Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules
Thermo Scientific Production Chemicals and Services 50MM TRIS 10 MM EDTA 1.65M AM
NC3588257 50MM TRIS 10 MM EDTA 1.65M AM