Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
GenDEPOT TBST(10X), pH 7.4 | 1L
TBST(10X) is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays. The 10X TBST (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.14M NaCl, 0.1% Tween-20, pH 7.4, when diluted to 1X with water.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 25 G
MOPS sodium salt is a buffering agent utilized in biological applications. MOPS buffer is effective in maintaining the pH of mammalian cell culture media and has been shown to significantly improve the thermal stability of BSA. It is relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Buffering agent for biological research.
- Maintains pH in mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Can be used in the preparation of keratinocyte and microbial culture media.
Medchemexpress LLC 1,4-Piperazinedipropanesulfonic acid, β1,4-dihydroxy- | 68189-43-5 | ≥95.0% | 25 G
POPSO is a zwitterionic buffer that increases osmolality and demonstrates significant inhibition of anion uniport. It inhibits chloride uniport with an IC50 value of 24 mM. POPSO also enhances copper uptake and toxicity in alga and impairs the mitochondrial inner membrane. The working pH range for POPSO sesquisodium salt is 7.2-8.5.
- Zwitterionic buffer
- Increases osmolality
- Marked inhibition of anion uniport
- Inhibits chloride uniport with an IC50 value of 24 mM
- Enhances copper uptake and toxicity in alga
- Impairs mitochondrial inner membrane
- Working pH range (for sesquisodium salt): 7.2-8.5
![Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000414468-logo.png-250.jpg){kind=logo}
Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G
TAPSO is a common biological buffer and pH stabilization reagent. It has an effective pH ranging from 7-8 and pKa values ranging from 7.5-9.0. This compound contains Tris groups and exhibits reactive activity with zwitterionic glycine peptides.
- Common biological buffer
- pH stabilization reagent
- Effective pH ranging from 7-8
- pKa values ranging from 7.5-9.0
- Contains Tris groups
- Reactive with zwitterionic glycine peptides
- Suitable for research applications
VWR INTERNATIONAL LLC TRIS-Borate-EDTA Buffer, 10X LIQUID CONCENTRATE, Ultra Pure Grade4l
TRIS-Borate-EDTA Buffer, 10X LIQUID CONCENTRATE, Ultra Pure Grade | 4l
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid >=98.0% (KT) | 60-00-4 | MFCD00003541 | 50G
Ethylenediaminetetraacetic acid >=98.0% (KT) | Purity: >=98.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 50G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 5kg
Ethylenediaminetetraacetic acid | Purity: ≥98.5%, powder | MW:292.24 | 60-00-4 | MFCD00003541 | 5kg
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, particularly in the preparation of enzyme buffers.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Especially useful in the preparation of enzyme buffers
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 25 g
MES monohydrate | Purity: ≥99.0% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 25 g
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 250G
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 250G
Medchemexpress LLC 4-Morpholinepropanesulfonic acid, sodium salt (1:1) | 71119-22-7 | 99.8% | 50 G
MOPS sodium salt is a buffering agent utilized in biological applications. It effectively maintains the pH of mammalian cell culture media. Additionally, MOPS sodium salt has been shown to significantly enhance the thermal stability of BSA (Bovine Serum Albumin).
- Buffering agent for biological applications.
- Maintains the pH of mammalian cell culture media.
- Significantly enhances the thermal stability of BSA.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG